bundle_3-probe-affinity

Three-Probe Bone Affinity Sampler

$999

Catalogue number: BV355013

This sampler kit includes three imaging probes with different spectroscopic properties and high, medium and low bone affinities (in the order of 5(6)-ROX-RIS > 5(6)-FAM-dRIS > AF647-RISPC). All three probes include bisphosphonate or phosphonocarboxylate molecular skeleton, thus have higher affinity than calcein (the fluorescent molecule with the least bone affinity). The kit can be used for simultaneous detection with co-dosing and studies on effects of bone affinity.

Kit Contents:

  • 24 nmol of 5(6)-ROX-RIS
  • 24 nmol of 5(6)-FAM-dRIS
  • 24 nmol of AF647-RISPC
Please contact us for more details about the sampler kit: inquiry@biovinc.com

 

Product Description

This sampler kit includes three imaging probes with different spectroscopic properties and high, medium and low bone affinities (in the order of 5(6)-ROX-RIS > 5(6)-FAM-dRIS > AF647-RISPC). All three probes include bisphosphonate or phosphonocarboxylate molecular skeleton, thus have higher affinity than calcein (the fluorescent molecule with the least bone affinity). The kit can be used for simultaneous detection with co-dosing and studies on effects of bone affinity.

 

Spectroscopic properties of 5(6)-ROX-RIS:
Maximum absorption wavelength (λmax (abs)): 580 nm
Maximum emission wavelength (λmax (em)): 605 nm
Extinction coefficient (M-1cm-1):  73,000 (0.1 M phosphate buffer, pH 8.0)

 

Spectroscopic properties of 5(6)-FAM-dRIS:
Maximum absorption wavelength (λmax (abs)): 493 nm
Maximum emission wavelength (λmax (em)): 518 nm
Extinction coefficient (M-1cm-1):  73,000 (0.1 M phosphate buffer, pH 7.2)
Spectroscopic properties of AF647-RISPC:
Maximum absorption wavelength (λmax (abs)): 648 nm
Maximum emission wavelength (λmax (em)): 666 nm
Extinction coefficient (M-1cm-1): 240,000 (0.1 M phosphate buffer, pH 7.0)

 

*For laboratory use only. These products are intended for animal research only and not for use in humans.

Spectroscopic Properties



Emission spectra of 5(6)-ROX-RIS (in 0.1 M phosphate buffer, pH 8.0), 5(6)-FAM-dRIS (in 0.1 M phosphate buffer, pH 7.2) and AF647-RISPC (in 0.1 M phosphate buffer, pH 7.0).

More Info

References

  • Fluorescent risedronate analogues reveal bisphosphonate uptake by bone marrow monocytes and localization around osteocytes in vivo. Roelofs, Anke J.; Coxon, Fraser P.; Ebetino, Frank H.; Lundy, Mark W.; Henneman, Zachary J.; Nancollas, George H.; Sun, Shuting; Blazewska, Katarzyna M.; Bala, Joy Lynn F.; Kashemirov, Boris A.; Khalid, Aysha B.; McKenna, Charles E.; Rogers, Michael J. 2010, Journal of Bone and Mineral Research,  25(3):  606-616.
  • Analysing the skeletal distribution of fluorescently-labelled bisphosphonates and lower affinity analogues in vivo. Roelofs, AJ, Boyde, Ebetino FH,A, Lundy, MW, McKenna CE, Blazewska, K, Sun, ST, Kashemirov, BA, Henneman, Z, Nancollas, GH, Russell RGG, Rogers, MJ, Coxon, FP. 2012 Journal of Bone and Mineral Res.  27(4): 835-847.
  • Equilibrium-dependant bisphosphonate interaction with crystalline bone mineral explains anti-resorptive pharmacokinetics and prevalenc of osteonecrosis oft he jaw in rats. Hogoku, A, Sun, S, Park, S, McKenna, CE, Nishimura, I. 2013 Bone 53:59-68.